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curve auc values  (ATCC)
98
ATCC curve auc values
Bacteriocin presence and inhibitory activity <t>of</t> <t>non-O157:H7</t> E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. <t>AUC</t> values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
Curve Auc Values, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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receiver operator curve auc values  (Genovis Inc)
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Genovis Inc receiver operator curve auc values
Bacteriocin presence and inhibitory activity <t>of</t> <t>non-O157:H7</t> E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. <t>AUC</t> values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
Receiver Operator Curve Auc Values, supplied by Genovis Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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auc values  (STATA Corporation)
99
STATA Corporation auc values
Bacteriocin presence and inhibitory activity <t>of</t> <t>non-O157:H7</t> E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. <t>AUC</t> values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
Auc Values, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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auc values  (ATCC)
96
ATCC auc values
Bacteriocin presence and inhibitory activity <t>of</t> <t>non-O157:H7</t> E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. <t>AUC</t> values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
Auc Values, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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curve auc values  (STATA Corporation)
99
STATA Corporation curve auc values
Bacteriocin presence and inhibitory activity <t>of</t> <t>non-O157:H7</t> E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. <t>AUC</t> values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
Curve Auc Values, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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selection operator vif variance inflation factor ppv positive predictive value npv negative predictive value roc receiver operating characteristic auc  (Genovis Inc)
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Genovis Inc selection operator vif variance inflation factor ppv positive predictive value npv negative predictive value roc receiver operating characteristic auc
Bacteriocin presence and inhibitory activity <t>of</t> <t>non-O157:H7</t> E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. <t>AUC</t> values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
Selection Operator Vif Variance Inflation Factor Ppv Positive Predictive Value Npv Negative Predictive Value Roc Receiver Operating Characteristic Auc, supplied by Genovis Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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receiver operator curve roc auc values  (Genovis Inc)
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Genovis Inc receiver operator curve roc auc values
Bacteriocin presence and inhibitory activity <t>of</t> <t>non-O157:H7</t> E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. <t>AUC</t> values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
Receiver Operator Curve Roc Auc Values, supplied by Genovis Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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receiver operator curve auc value  (Genovis Inc)
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Bacteriocin presence and inhibitory activity <t>of</t> <t>non-O157:H7</t> E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. <t>AUC</t> values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
Receiver Operator Curve Auc Value, supplied by Genovis Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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receiver operator curve auc value - by Bioz Stars, 2026-04
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1000 auc values  (Unilife Inc)
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Bacteriocin presence and inhibitory activity <t>of</t> <t>non-O157:H7</t> E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. <t>AUC</t> values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
1000 Auc Values, supplied by Unilife Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

Journal: Microorganisms

Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

doi: 10.3390/microorganisms13122811

Figure Lengend Snippet: Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

Article Snippet: Area under the curve (AUC) values of E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) exposed to heat-inactivated, cell-free supernatants from unique bovine non-O157:H7 E. coli strains; Table S5.

Techniques: Activity Assay, In Vitro, Inhibition

Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

Journal: Microorganisms

Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

doi: 10.3390/microorganisms13122811

Figure Lengend Snippet: Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

Article Snippet: Area under the curve (AUC) values of E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) exposed to heat-inactivated, cell-free supernatants from unique bovine non-O157:H7 E. coli strains; Table S5.

Techniques:

Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

Journal: Microorganisms

Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

doi: 10.3390/microorganisms13122811

Figure Lengend Snippet: Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

Article Snippet: Strains 14_437FEN5, 61_438REC, 69_426FEC, and 87_435FED produced significantly higher AUC values than both O157:H7 strains (ATCC 700728 and ATCC 43888) for all nutrients tested outside of ethanolamine, and were combined into a consortium which was deemed “highly competitive” (HC) for the nutrients tested.

Techniques: Activity Assay, In Vitro, Inhibition

Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

Journal: Microorganisms

Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

doi: 10.3390/microorganisms13122811

Figure Lengend Snippet: Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

Article Snippet: Strains 14_437FEN5, 61_438REC, 69_426FEC, and 87_435FED produced significantly higher AUC values than both O157:H7 strains (ATCC 700728 and ATCC 43888) for all nutrients tested outside of ethanolamine, and were combined into a consortium which was deemed “highly competitive” (HC) for the nutrients tested.

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